《植物生理学报》 2018, 54(1): 165-172
通信作者：许自成；E-mail: zichengxu@126.com
摘 要：
采用盆栽试验, 研究了外源硒(2~12 mg·kg-1)对干旱胁迫下烟株生长、叶片光合色素含量、光合特性、抗氧化酶活性以及渗透调节物质含量的影响。结果表明: 干旱胁迫抑制烤烟生长, 显著影响烤烟的生长和生理生化代谢的相关指标。低浓度的外源硒能不同程度的缓解烤烟所受干旱胁迫的伤害, 但高浓度外源硒处理则表现出毒害效应。适宜浓度的外源硒显著促进了干旱胁迫下烤烟植株生长, 提高了烟株叶片光合色素含量, 提高净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr), 降低胞间CO2浓度(Ci), 同时也提高了烤烟叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性以及可溶性蛋白、脯氨酸和可溶性糖等渗透调节物质含量, 降低了叶片丙二醛(MDA)的积累。其中4 mg·kg-1外源硒处理缓解效果最好。关键词：外源硒; 烤烟; 干旱胁迫; 生理特性
收稿：2017-09-19 修定：2017-12-11
资助：中国烟草总公司河南省公司重点科技攻关项目(HYKJ201405)。
Corresponding author: XU Zi-Cheng; E-mail: zichengxu@126.com
Abstract:
A pot experiment was conducted to study the effects of exogenous selenium (2–12 mg·kg-1) on plant growth, leaf photosynthetic pigment content, photosynthetic characteristics, antioxidant enzyme activities and osmotic adjustment substances in flue-cured tobacco leaves under drought stress. The result showed that drought stress inhibited the growth of tobacco and significantly affected the growth, physiological, biochemical and metabolism of tobacco. The lower exogenous selenium concentrations can alleviate the drought stress of tobacco and the higher exogenous selenium concentration had toxic effects on tobacco. Appropriate concentration of exogenous selenium concentrations significantly promoted the growth of tobacco, improved photosynthetic pigment content, net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr), elevated the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and increased the content of soluble protein, proline and soluble sugar, but reduced the intercellular CO2 concentration (Ci) and malondialdehyde (MDA) accumulation in tobacco leaves under drought stress. The strongest effect on alleviating drought stress was found in the treatment of 4 mg·kg-1 exogenous selenium.Key words: exogenous selenium; flue-cured tobacco; drought stress; physiological characteristics
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